GANAB antibody (N-Term)

Catalog No. ABIN2855336

The Rabbit Polyclonal anti-GANAB antibody has been validated for WB, IF, IHC (p) and ICC. It is suitable to detect GANAB in samples from Human and Mouse. There is 1 publication available.

Quick Overview for GANAB antibody (N-Term) (ABIN2855336)

Target: GANAB (Glucosidase, Alpha, Neutral AB (GANAB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GANAB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-GANAB Antibody

Binding Specificity: N-Term

Characteristics: Rabbit Polyclonal antibody to alpha Glucosidase II (glucosidase, alpha, neutral AB)
alpha Glucosidase II antibody [N1N2], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human alpha Glucosidase II. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-GANAB antibody (ABIN2855336)

Timms, Menzies, Tchasovnikarova, Christensen, Williamson, Antrobus, Dougan, Ellgaard, Lehner: "Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens." in: Nature communications, Vol. 7, pp. 11786, (2018) (PubMed).

Target Details for GANAB

Target: GANAB (Glucosidase, Alpha, Neutral AB (GANAB))

Alternative Name: alpha Glucosidase II

Background: Cellular Localization: Endoplasmic reticulum (By similarity) , Golgi apparatus (By similarity) , Melanosome

Molecular Weight: 107 kDa

Gene ID: 23193