EEF2 antibody

Catalog No. ABIN2855346

The Rabbit Polyclonal anti-EEF2 antibody (ABIN2855346) specifically detects EEF2 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for EEF2 antibody (ABIN2855346)

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EEF2 Antibody

Cross-Reactivity: Drosophila melanogaster, Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to EEF2 (eukaryotic translation elongation factor 2)
eEF2 antibody [N2C1], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human eEF2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , GL261 , NIH-3T3 , C2C12 , Neuro 2A , Raw264.7 , PC-12

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.86 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EEF2

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Alternative Name: eukaryotic translation elongation factor 2

Background: This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.

Cellular Localization: Cytoplasm

Molecular Weight: 95 kDa

Gene ID: 1938

UniProt: P13639

Pathways: AMPK Signaling