EEF1A2 antibody

Catalog No. ABIN2855358

This anti-EEF1A2 antibody is a Rabbit Polyclonal antibody detecting EEF1A2 in WB and IHC (p). Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for EEF1A2 antibody (ABIN2855358)

Target: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF1A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-EEF1A2 Antibody

Specificity: Based on a BLAST result, it may also cross with human EEF1A1.

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to EEF1A2 (eukaryotic translation elongation factor 1 alpha 2)
EEF1A2 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human EEF1A2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , mouse muscle , rat brain , 293T

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.21 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-EEF1A2 antibody (ABIN2855358)

Losada, Muñoz-Alonso, García, Sánchez-Murcia, Martínez-Leal, Domínguez, Lillo, Gago, Galmarini: "Translation Elongation Factor eEF1A2 is a Novel Anticancer Target for the Marine Natural Product Plitidepsin." in: Scientific reports, Vol. 6, pp. 35100, (2018) (PubMed).

Target Details for EEF1A2

Target: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Alternative Name: eukaryotic translation elongation factor 1 alpha 2

Background: This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Nucleus

Molecular Weight: 50 kDa

Gene ID: 1917

UniProt: Q05639