EEF1A2 antibody (AA 46-72)

Catalog No. ABIN390520

This Rabbit Polyclonal antibody specifically detects EEF1A2 in WB, IHC (p) and FACS. It exhibits reactivity toward Human. It has been mentioned in 4+ publications

Quick Overview for EEF1A2 antibody (AA 46-72) (ABIN390520)

Target: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF1A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: RB19607

Product Details anti-EEF1A2 Antibody

Binding Specificity: AA 46-72

Predicted Reactivity: X, B, Ha, D, Hs, M, Rb, Rat, E, C, Zf, Y

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids of human EEF1A1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-EEF1A2 antibody (ABIN390520)

Steiner, Lang: "Alcohol intoxication following muscle contraction in mice decreases muscle protein synthesis but not mTOR signal transduction." in: Alcoholism, clinical and experimental research, Vol. 39, Issue 1, pp. 1-10, (2015) (PubMed).

Boratkó, Péter, Thalwieser, Kovács, Csortos: "Elongation factor-1A1 is a novel substrate of the protein phosphatase 1-TIMAP complex." in: The international journal of biochemistry & cell biology, Vol. 69, pp. 105-113, (2015) (PubMed).

Vermillion, Lidberg, Gammill: "Cytoplasmic protein methylation is essential for neural crest migration." in: The Journal of cell biology, Vol. 204, Issue 1, pp. 95-109, (2014) (PubMed).

Descamps, Wattiez, Saussez: "Proteomic study of HPV-positive head and neck cancers: preliminary results." in: BioMed research international, Vol. 2014, pp. 430906, (2014) (PubMed).

Target Details for EEF1A2

Target: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Alternative Name: EEF1A1/ EEF1A2

Background: EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66 % of patients with Felty syndrome.

Molecular Weight: 50141

Gene ID: 1915

NCBI Accession: NP_001393

UniProt: P68104