MCCC2 antibody (Center)

Catalog No. ABIN2855455

This Rabbit Polyclonal antibody specifically detects MCCC2 in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for MCCC2 antibody (Center) (ABIN2855455)

Target: MCCC2 (Methylcrotonoyl-CoA Carboxylase 2 (Beta) (MCCC2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCCC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MCCC2 Antibody

Binding Specificity: Center

Cross-Reactivity: Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio), Xenopus tropicalis

Cross-Reactivity (Details): Rat (88 %), Xenopus laevis (84 %), Zebrafish (84 %), Xenopus tropicalis (85 %)

Characteristics: Rabbit Polyclonal antibody to MCCC2 (methylcrotonoyl-Coenzyme A carboxylase 2 (beta))
MCCC2 antibody [N1C1]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MCCC2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HepG2 , mouse liver

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MCCC2

Target: MCCC2 (Methylcrotonoyl-CoA Carboxylase 2 (Beta) (MCCC2))

Alternative Name: MCCC2

Background: This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.

Cellular Localization: Mitochondrion matrix

Molecular Weight: 61 kDa

Gene ID: 64087