POLD1 antibody (Center)

Catalog No. ABIN2855506

This Rabbit Polyclonal antibody specifically detects POLD1 in WB, ICC, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for POLD1 antibody (Center) (ABIN2855506)

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-POLD1 Antibody

Binding Specificity: Center

Cross-Reactivity: Human, Rat

Characteristics: Rabbit Polyclonal antibody to DNA polymerase delta (polymerase (DNA directed), delta 1, catalytic subunit 125 kDa)
DNA polymerase delta antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DNA polymerase delta. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Rat2 , Molt-4 , 293T , A431 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.56 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for POLD1

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Alternative Name: DNA polymerase delta 1

Background: The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA, MIM 176740), the multisubunit replication factor C (see MIM 102579), and the 4 subunit polymerase complex: POLD1, POLD2 (MIM 600815), POLD3 (MIM 611415), and POLD4 (MIM 611525) (Liu and Warbrick, 2006 [PubMed 16934752]).[supplied by OMIM]

Cellular Localization: Nucleus

Molecular Weight: 124 kDa

Gene ID: 5424

UniProt: P28340

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Chromatin Binding, Synthesis of DNA