POLD1 antibody (Center)
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- Target See all POLD1 Antibodies
- POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))
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Binding Specificity
- Center
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This POLD1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity
- Human, Rat
- Characteristics
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Rabbit Polyclonal antibody to DNA polymerase delta (polymerase (DNA directed), delta 1, catalytic subunit 125 kDa)
DNA polymerase delta antibody - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human DNA polymerase delta. The exact sequence is proprietary.
- Isotype
- IgG
- Top Product
- Discover our top product POLD1 Primary Antibody
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- Application Notes
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Comment
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Positive Control: Rat2 , Molt-4 , 293T , A431 , HeLa , HepG2
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.56 mg/mL
- Buffer
- 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))
- Alternative Name
- DNA polymerase delta 1 (POLD1 Products)
- Background
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The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA, MIM 176740), the multisubunit replication factor C (see MIM 102579), and the 4 subunit polymerase complex: POLD1, POLD2 (MIM 600815), POLD3 (MIM 611415), and POLD4 (MIM 611525) (Liu and Warbrick, 2006 [PubMed 16934752]).[supplied by OMIM]
Cellular Localization: Nucleus - Molecular Weight
- 124 kDa
- Gene ID
- 5424
- UniProt
- P28340
- Pathways
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Chromatin Binding, Synthesis of DNA
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