XPA antibody (Center)

Catalog No. ABIN2855566

This Rabbit Polyclonal antibody specifically detects XPA in WB, IF, IHC (p), IP and ICC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for XPA antibody (Center) (ABIN2855566)

Target: XPA (Xeroderma Pigmentosum, Complementation Group A (XPA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XPA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-XPA Antibody

Binding Specificity: Center

Characteristics: Rabbit Polyclonal antibody to XPA (xeroderma pigmentosum, complementation group A)
XPA antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human XPA. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:500-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:500-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: A549

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-XPA antibody (ABIN2855566)

Manandhar, Lowery, Boulware, Lin, Lu, Wood: "Transcriptional consequences of XPA disruption in human cell lines." in: DNA repair, Vol. 57, pp. 76-90, (2017) (PubMed).

Target Details for XPA

Target: XPA (Xeroderma Pigmentosum, Complementation Group A (XPA))

Alternative Name: XPA

Background: This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene.

Cellular Localization: Nucleus

Molecular Weight: 31 kDa

Gene ID: 7507

Pathways: DNA Damage Repair