MSH3 antibody (N-Term)

Catalog No. ABIN2855583

This anti-MSH3 antibody is a Rabbit Polyclonal antibody detecting MSH3 in WB. Suitable for Human.

Quick Overview for MSH3 antibody (N-Term) (ABIN2855583)

Target: MSH3 (MutS Homolog 3 (MSH3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSH3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MSH3 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Cow (Bovine)

Cross-Reactivity (Details): Bovine (83 %)

Characteristics: Rabbit Polyclonal antibody to MSH3 (mutS homolog 3 (E. coli))
MSH3 antibody [N1N2], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human MSH3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: A549 , H1299 , HCT116

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MSH3

Target: MSH3 (MutS Homolog 3 (MSH3))

Alternative Name: MSH3

Background: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.

Molecular Weight: 127 kDa

Gene ID: 4437

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response