RAD54B antibody (C-Term)

Catalog No. ABIN2855606

The Rabbit Polyclonal anti-RAD54B antibody has been validated for WB, IF, IHC (p) and ICC. It is suitable to detect RAD54B in samples from Human. There is 1 publication available.

Quick Overview for RAD54B antibody (C-Term) (ABIN2855606)

Target: RAD54B (DNA repair and recombination protein RAD54B (RAD54B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD54B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-RAD54B Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to Rad54B (RAD54 homolog B (S. cerevisiae))
Rad54B antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human RAD54B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Molt-4

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-RAD54B antibody (ABIN2855606)

Chen, Tsai, Wang, Teng: "SMYD3 Promotes Homologous Recombination via Regulation of H3K4-mediated Gene Expression." in: Scientific reports, Vol. 7, Issue 1, pp. 3842, (2017) (PubMed).

Target Details for RAD54B

Target: RAD54B (DNA repair and recombination protein RAD54B (RAD54B))

Alternative Name: RAD54 homolog B

Background: The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.

Cellular Localization: Nucleus

Gene ID: 25788

UniProt: Q9Y620, O95073