C-MYC antibody (Myc Proto-Oncogene protein) (Center)

Details for Product anti-MYC Antibody No. ABIN2855631
Target
  • MRTL
  • MYCC
  • bHLHe39
  • c-Myc
  • CMYC
  • c-myc
  • C-MYC
  • LOC100136746
  • RNCMYC
  • mMyc
  • AU016757
  • Myc2
  • Niard
  • Nird
  • MYC
  • cmyc
  • zc-myc
  • c-myc II
  • myc-B
  • myc2
  • CMyc
  • CG10798
  • D-Myc
  • DM
  • DMYc
  • Dm
  • Dmel\CG10798
  • Dmyc
  • EG:BACN5I9.1
  • anon-WO03040301.171
  • bHLHe57
  • c-MYC
  • d-myc
  • dMYC
  • dMyc
  • dMyc1
  • da
  • dm
  • dm/dMyc
  • dm/myc
  • dmyc
  • dmyc1
  • l(1)G0139
  • l(1)G0354
  • l(1)G0359
  • myc
  • MYC proto-oncogene, bHLH transcription factor
  • v-myc myelocytomatosis viral oncogene homolog
  • myelocytomatosis oncogene
  • MYC proto-oncogene, bHLH transcription factor a
  • MYC proto-oncogene, bHLH transcription factor L homeolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)
  • CG10798 gene product from transcript CG10798-RB
  • MYC
  • LOC100136746
  • Myc
  • myca
  • myc.L
Binding Specificity
Center
79
54
43
42
28
24
24
22
16
15
15
11
11
10
9
9
8
5
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse
492
159
137
17
17
15
14
8
8
8
7
6
5
5
4
4
4
3
3
2
2
2
2
1
1
1
1
1
Host
Rabbit
327
158
17
11
5
1
Clonality
Polyclonal
Conjugate
This C-MYC antibody is un-conjugated
41
33
28
10
10
10
8
7
7
7
7
7
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
341
164
159
118
102
77
73
52
35
29
18
18
14
13
8
5
4
4
3
2
2
Options
Immunogen Recombinant protein encompassing a sequence within the center region of human c-Myc. The exact sequence is proprietary.
Isotype IgG
Cross-Reactivity Chimpanzee
Cross-Reactivity (Details) Chimpanzee (100 %)
Characteristics Rabbit Polyclonal antibody to c-Myc (v-myc myelocytomatosis viral oncogene homolog (avian))
c-Myc antibody
Purification Purified by antigen-affinity chromatography.
Target
Alternative Name C-Myc (MYC Antibody Abstract)
Background The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.

Cellular Localization: Nucleus
Molecular Weight 49 kDa
Gene ID 4609
Pathways p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus
Application Notes Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: 293T , NIH-3T3 , Raw264.7 , HeLa , HeLa nuclear extract

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Images
Image no. 1 for anti-Myc Proto-Oncogene protein (MYC) (Center) antibody (ABIN2855631) WB Image Sample (30 ug of whole cell lysate)    A: NIH-3T3   B: Raw264.7   10% SDS PA...
Image no. 2 for anti-Myc Proto-Oncogene protein (MYC) (Center) antibody (ABIN2855631) WB Image Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug 10% SDS PAGE antibody d...
Image no. 3 for anti-Myc Proto-Oncogene protein (MYC) (Center) antibody (ABIN2855631) ICC/IF Image c-Myc antibody detects c-Myc protein at nucleus by immunofluorescent ana...
Image no. 4 for anti-Myc Proto-Oncogene protein (MYC) (Center) antibody (ABIN2855631) IP Image c-Myc antibody immunoprecipitates c-Myc protein in IP experiments. IP Sample...
Image no. 5 for anti-Myc Proto-Oncogene protein (MYC) (Center) antibody (ABIN2855631) WB Image c-Myc antibody detects c-Myc protein by western blot analysis. HeLa whole ce...
Image no. 6 for anti-Myc Proto-Oncogene protein (MYC) (Center) antibody (ABIN2855631) ChIP Image c-Myc antibody immunoprecipitates c-Myc protein-DNA in ChIP experiments. C...
Product cited in: Chen, Ding, Chen, Hu, Lu, Wu, Zhang, Yang, Wu, Peng, Xie, Zhou, Wu, Zheng: "ACSL4 promotes hepatocellular carcinoma progression via c-Myc stability mediated by ERK/FBW7/c-Myc axis." in: Oncogenesis, Vol. 9, Issue 4, pp. 42, 2020 (PubMed).

Seira, Yamagata, Fukushima, Araki, Kurata, Yanagisawa, Mashimo, Nakamura, Regan, Murayama, Fujino: "Cellular density-dependent increases in HIF-1α compete with c-Myc to down-regulate human EP4 receptor promoter activity through Sp-1-binding region." in: Pharmacology research & perspectives, Vol. 6, Issue 6, pp. e00441, 2019 (PubMed).

Ma, Wang, Chen, Li, Hur, Saijilafu: "Differential Roles of Glycogen Synthase Kinase 3 Subtypes Alpha and Beta in Cortical Development." in: Frontiers in molecular neuroscience, Vol. 10, pp. 391, 2017 (PubMed).

Chung, Liu, Lin, Chi, Huang, Yang, Yeh, Tan, Lin: "ChIP-on-chip analysis of thyroid hormone-regulated genes and their physiological significance." in: Oncotarget, Vol. 7, Issue 16, pp. 22448-59, 2017 (PubMed).

Wu, Lee, Hua, Kuo, Lin: "Macrophage Infiltration Induces Gastric Cancer Invasiveness by Activating the β-Catenin Pathway." in: PLoS ONE, Vol. 10, Issue 7, pp. e0134122, 2016 (PubMed).