Seladin 1 antibody

Catalog No. ABIN2855685

The Rabbit Polyclonal anti-Seladin 1 antibody (ABIN2855685) specifically detects Seladin 1 in WB, IHC (p), IF and ICC. The antibody is reactive with Human samples.

Quick Overview for Seladin 1 antibody (ABIN2855685)

Target: Seladin 1 (DHCR24) (24-Dehydrocholesterol Reductase (DHCR24))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Seladin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Seladin 1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to Seladin 1 (24-dehydrocholesterol reductase)
Seladin 1 antibody [N1C2]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Seladin 1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.47 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Seladin 1

Target: Seladin 1 (DHCR24) (24-Dehydrocholesterol Reductase (DHCR24))

Alternative Name: 24-dehydrocholesterol reductase

Background: This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.

Cellular Localization: Endoplasmic reticulum membrane , Golgi apparatus membrane

Molecular Weight: 60 kDa

Gene ID: 1718

UniProt: Q15392