GNAQ antibody (Center)

Catalog No. ABIN2855848

This Rabbit Polyclonal antibody specifically detects GNAQ in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for GNAQ antibody (Center) (ABIN2855848)

Target: GNAQ (Guanine Nucleotide Binding Protein (G Protein), Q Polypeptide (GNAQ))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNAQ antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GNAQ Antibody

Binding Specificity: Center

Cross-Reactivity: Dog (Canine), Pig (Porcine), Cow (Bovine), Rhesus Monkey

Cross-Reactivity (Details): Dog (100 %), Pig (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Characteristics: Rabbit polyclonal antibody to GNAQ (guanine nucleotide binding protein (G protein), q polypeptide)
GNAQ antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human GNAQ. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 3xFlag-human GNAQ-transfected 293T

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-GNAQ antibody (ABIN2855848)

Rushton, Korb, Kummer, Reichart, Fuchs-Baumgartinger, Tichy, Nell: "Protein expression of KIT, BRAF, GNA11, GNAQ and RASSF1 in feline diffuse iris melanomas." in: Veterinary journal (London, England : 1997), Vol. 249, pp. 33-40, (2019) (PubMed).

Target Details for GNAQ

Target: GNAQ (Guanine Nucleotide Binding Protein (G Protein), Q Polypeptide (GNAQ))

Alternative Name: GNAQ

Background: Guanine nucleotide-binding proteins are a family of heterotrimeric proteins that couple cell surface, 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit. G-alpha-q is the alpha subunit of one of the heterotrimeric GTP-binding proteins that mediates stimulation of phospholipase C-beta (MIM 600230).[supplied by OMIM]

Molecular Weight: 42 kDa

Gene ID: 2776

Pathways: JAK-STAT Signaling, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction