RICTOR antibody (C-Term)

Catalog No. ABIN2855866

This Rabbit Polyclonal antibody specifically detects RICTOR in WB, IHC, IHC (p), IF and ICC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for RICTOR antibody (C-Term) (ABIN2855866)

Target: RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RICTOR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RICTOR Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to RICTOR
RICTOR antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RICTOR. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-RICTOR antibody (ABIN2855866)

Liu, Zhang, Li, Cao, Wan, Xi, Tan, Wang, Yang, Feng, Ye, Chen, Wu, Xi, Wang, Zhou, Feng, Ma, Gao: "C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation." in: Nature communications, Vol. 9, Issue 1, pp. 1739, (2018) (PubMed).

Target Details for RICTOR

Target: RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))

Alternative Name: RPTOR independent companion of MTOR complex 2

Background: RICTOR and MTOR (FRAP1, MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM]

Molecular Weight: 192 kDa

Gene ID: 253260

UniProt: Q6R327

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events