POLR2A/RPB1 antibody (C-Term)

Catalog No. ABIN2855904

This anti-POLR2A/RPB1 antibody is a Rabbit Polyclonal antibody detecting POLR2A/RPB1 in WB. Suitable for Human.

Quick Overview for POLR2A/RPB1 antibody (C-Term) (ABIN2855904)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2A/RPB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR2A/RPB1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Mouse (Murine), Cow (Bovine)

Cross-Reactivity (Details): Mouse (100 %), Bovine (100 %)

Characteristics: Rabbit polyclonal antibody to POLR2A (polymerase (RNA) II (DNA directed) polypeptide A, 220 kDa)
RPB1 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RPB1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HepG2 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.72 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: POLR2A

Background: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Cellular Localization: Nucleus

Molecular Weight: 217 kDa

Gene ID: 5430

Pathways: Regulatory RNA Pathways