ADAM10 antibody

Catalog No. ABIN2855998

This Rabbit Polyclonal antibody specifically detects ADAM10 in WB, ICC, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for ADAM10 antibody (ABIN2855998)

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM10 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ADAM10 Antibody

Cross-Reactivity: Drosophila melanogaster, Human, Mouse

Characteristics: Rabbit Polyclonal antibody to ADAM10 (disintegrin and metalloproteinase domain-containing protein 10)
ADAM10 antibody [N2C1], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ADAM10. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: THP-1

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.48 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ADAM10

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Alternative Name: ADAM metallopeptidase domain 10

Background: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin.

Cellular Localization: Cell membrane , Endomembrane system

Molecular Weight: 84 kDa

Gene ID: 102

UniProt: O14672

Pathways: Notch Signaling, EGFR Signaling Pathway