alpha KGDHC antibody (C-Term)

Catalog No. ABIN2856043

This Rabbit Polyclonal antibody specifically detects alpha KGDHC in WB, IF, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for alpha KGDHC antibody (C-Term) (ABIN2856043)

Target: alpha KGDHC (alphaKGDHC) (alpha Ketoglutarate Dehydrogenase (alphaKGDHC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha KGDHC antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-alpha KGDHC Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to OGDH (oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide))
OGDH antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human OGDH. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , 293T , A431 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for alpha KGDHC

Target: alpha KGDHC (alphaKGDHC) (alpha Ketoglutarate Dehydrogenase (alphaKGDHC))

Alternative Name: oxoglutarate dehydrogenase

Background: This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitocondrial matrix and uses thiamine pyrophosphate as a cofactor. A congential deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia.

Cellular Localization: Mitochondrion matrix

Molecular Weight: 116 kDa

Gene ID: 4967

UniProt: Q02218