POLRMT antibody (Center)

Catalog No. ABIN2856044

The Rabbit Polyclonal anti-POLRMT antibody has been validated for WB, IF, ICC, IHC (p) and CUT&RUN. It is suitable to detect POLRMT in samples from Human and Mouse. There are 3+ publications available.

Quick Overview for POLRMT antibody (Center) (ABIN2856044)

Target: POLRMT (Polymerase (RNA) Mitochondrial (DNA Directed) (POLRMT))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLRMT antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Product Details anti-POLRMT Antibody

Binding Specificity: Center

Characteristics: Rabbit Polyclonal antibody to mtRNA polymerase (polymerase (RNA) mitochondrial (DNA directed))
mtRNA polymerase antibody [N2N3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human mtRNA polymerase. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.31 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-POLRMT antibody (ABIN2856044)

Kasinathan, Henikoff: "Non-B-Form DNA Is Enriched at Centromeres." in: Molecular biology and evolution, Vol. 35, Issue 4, pp. 949-962, (2019) (PubMed).

Thakur, Henikoff: "Unexpected conformational variations of the human centromeric chromatin complex." in: Genes & development, Vol. 32, Issue 1, pp. 20-25, (2018) (PubMed).

Sobek, Dalla Rosa, Pommier, Bornholz, Kalfalah, Zhang, Wiesner, von Kleist-Retzow, Hillebrand, Schaal, Mielke, Christensen, Boege: "Negative regulation of mitochondrial transcription by mitochondrial topoisomerase I." in: Nucleic acids research, Vol. 41, Issue 21, pp. 9848-57, (2014) (PubMed).

Target Details for POLRMT

Target: POLRMT (Polymerase (RNA) Mitochondrial (DNA Directed) (POLRMT))

Alternative Name: MtRNA Polymerase

Background: This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes.

Cellular Localization: Mitochondrion

Molecular Weight: 139 kDa

Gene ID: 5442