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POLRMT antibody (Center)

POLRMT Reactivity: Human, Mouse WB, IF, ICC, IHC (p), CUT&RUN Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2856044
  • Target See all POLRMT Antibodies
    POLRMT (Polymerase (RNA) Mitochondrial (DNA Directed) (POLRMT))
    Binding Specificity
    • 15
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Center
    Reactivity
    • 29
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 23
    • 5
    • 1
    Rabbit
    Clonality
    • 26
    • 3
    Polyclonal
    Conjugate
    • 12
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This POLRMT antibody is un-conjugated
    Application
    • 21
    • 13
    • 13
    • 11
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
    Characteristics
    Rabbit Polyclonal antibody to mtRNA polymerase (polymerase (RNA) mitochondrial (DNA directed))
    mtRNA polymerase antibody [N2N3]
    Purification
    Purified by antigen-affinity chromatography.
    Immunogen
    Recombinant protein encompassing a sequence within the center region of human mtRNA polymerase. The exact sequence is proprietary.
    Isotype
    IgG
    Top Product
    Discover our top product POLRMT Primary Antibody
  • Application Notes
    Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
    Comment

    Positive Control: HepG2

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.31 mg/mL
    Buffer
    0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Kasinathan, Henikoff: "Non-B-Form DNA Is Enriched at Centromeres." in: Molecular biology and evolution, Vol. 35, Issue 4, pp. 949-962, (2019) (PubMed).

    Thakur, Henikoff: "Unexpected conformational variations of the human centromeric chromatin complex." in: Genes & development, Vol. 32, Issue 1, pp. 20-25, (2018) (PubMed).

    Sobek, Dalla Rosa, Pommier, Bornholz, Kalfalah, Zhang, Wiesner, von Kleist-Retzow, Hillebrand, Schaal, Mielke, Christensen, Boege: "Negative regulation of mitochondrial transcription by mitochondrial topoisomerase I." in: Nucleic acids research, Vol. 41, Issue 21, pp. 9848-57, (2014) (PubMed).

  • Target
    POLRMT (Polymerase (RNA) Mitochondrial (DNA Directed) (POLRMT))
    Alternative Name
    MtRNA Polymerase (POLRMT Products)
    Synonyms
    polrmt-a antibody, mtRPO antibody, wu:fb73g10 antibody, si:dkeyp-74c5.5 antibody, POLRMT antibody, APOLMT antibody, MTRNAP antibody, MTRPOL antibody, h-mtRPOL antibody, 1110018N15Rik antibody, 4932416K13 antibody, RNA polymerase mitochondrial antibody, polymerase (RNA) mitochondrial (DNA directed) L homeolog antibody, polymerase (RNA) mitochondrial (DNA directed) antibody, Polrmt antibody, polrmt.L antibody, polrmt antibody, POLRMT antibody
    Background
    This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes.

    Cellular Localization: Mitochondrion
    Molecular Weight
    139 kDa
    Gene ID
    5442
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