MBD2 antibody (Center)

Catalog No. ABIN2856140

The Rabbit Polyclonal anti-MBD2 antibody has been validated for WB. It is suitable to detect MBD2 in samples from Human.

Quick Overview for MBD2 antibody (Center) (ABIN2856140)

Target: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MBD2 Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Xenopus laevis

Cross-Reactivity (Details): Mouse (98 %), Rat (98 %), Xenopus laevis (89 %)

Characteristics: Rabbit polyclonal antibody to MBD2 (methyl-CpG binding domain protein 2)
MBD2 antibody [N2C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MBD2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: A431 , Jurkat , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.48 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MBD2

Target: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Alternative Name: MBD2

Background: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.

Cellular Localization: Nucleus

Molecular Weight: 43 kDa

Gene ID: 8932

Pathways: Chromatin Binding