Cytokeratin 18 antibody
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- Target See all Cytokeratin 18 (KRT18) Antibodies
- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Cytokeratin 18 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Cross-Reactivity
- Dog, Human, Mouse
- Characteristics
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Rabbit polyclonal antibody to Cytokeratin 18 (keratin 18)
Cytokeratin 18 antibody [N2C2], Internal - Purification
- Purified by antigen-affinity chromatography.
- Grade
- KO Validated
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human Cytokeratin 18. The exact sequence is proprietary.
- Isotype
- IgG
- Top Product
- Discover our top product KRT18 Primary Antibody
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- Application Notes
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Comment
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Positive Control: H1299 , HepG2 , HeLa
Validation: KO/KD
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.54 mg/mL
- Buffer
- 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
- Alternative Name
- keratin 18 (KRT18 Products)
- Background
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KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Cellular Localization: Cytoplasm , perinuclear region - Molecular Weight
- 48 kDa
- Gene ID
- 3875
- UniProt
- P05783
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis
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