NCKAP1 antibody

Catalog No. ABIN2856158

The Rabbit Polyclonal anti-NCKAP1 antibody has been validated for WB and IHC (fro). It is suitable to detect NCKAP1 in samples from Human.

Quick Overview for NCKAP1 antibody (ABIN2856158)

Target: NCKAP1 (NCK Associated Protein 1 (NCKAP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCKAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NCKAP1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to NCKAP1 (NCK-associated protein 1)
NCKAP1 antibody [C1C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NCKAP1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , rat brain , NCKAP1-transfected 293T

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NCKAP1

Target: NCKAP1 (NCK Associated Protein 1 (NCKAP1))

Alternative Name: NCK associated protein 1

Background: Is part of lamellipodial complex that controls Rac-dependent actin remodeling.

Cellular Localization: Cell membrane, Single-pass membrane protein, Cytoplasmic side (By similarity) , Cell projection , lamellipodium membrane, Single-pass membrane protein, Cytoplasmic side (By similarity)

Molecular Weight: 129 kDa

Gene ID: 10787

UniProt: Q9Y2A7

Pathways: RTK Signaling, Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis