PFKL antibody (Center)

Catalog No. ABIN2856164

The Rabbit Polyclonal anti-PFKL antibody has been validated for WB, IF, IHC (p) and ICC. It is suitable to detect PFKL in samples from Human and Mouse.

Quick Overview for PFKL antibody (Center) (ABIN2856164)

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKL antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-PFKL Antibody

Binding Specificity: Center

Cross-Reactivity: Cow (Bovine), Rat (Rattus)

Cross-Reactivity (Details): Rat (95 %), Bovine (94 %)

Characteristics: Rabbit Polyclonal antibody to PFKL (phosphofructokinase, liver)
PFKL antibody [C1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PFKL. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: A431 , H1299 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.8 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PFKL

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Alternative Name: PFKL

Background: Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD).

Molecular Weight: 85 kDa

Gene ID: 5211

Pathways: Negative Regulation of Hormone Secretion, Warburg Effect