NFIB antibody

Catalog No. ABIN2856178

This Rabbit Polyclonal antibody specifically detects NFIB in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for NFIB antibody (ABIN2856178)

Target: NFIB (Nuclear Factor I/B (NFIB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NFIB Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to NFIB (nuclear factor I/B)
NFIB antibody [N1C2]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NFIB. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , NFIB transfected 293T

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.57 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NFIB

Target: NFIB (Nuclear Factor I/B (NFIB))

Alternative Name: nuclear factor I B

Background: Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Cellular Localization: Nucleus

Molecular Weight: 47 kDa

Gene ID: 4781

UniProt: O00712