RAE1 antibody (C-Term)

Catalog No. ABIN2856302

The Rabbit Polyclonal anti-RAE1 antibody has been validated for WB, IF and ICC. It is suitable to detect RAE1 in samples from Human.

Quick Overview for RAE1 antibody (C-Term) (ABIN2856302)

Target: RAE1 (RAE1 RNA Export 1 Homolog (S. Pombe) (RAE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RAE1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio), Xenopus laevis, Xenopus tropicalis, Pig (Porcine), Cow (Bovine)

Cross-Reactivity (Details): Mouse (100 %), Rat (100 %), Zebrafish (100 %), Xenopus laevis (100 %), Xenopus Tropicalis (100 %), Pig (100 %), Bovine (100 %)

Characteristics: Rabbit polyclonal antibody to RAE1 (RAE1 RNA export 1 homolog (S. pombe))
RAE1 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RAE1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: HeLaS3 , Molt-4 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RAE1

Target: RAE1 (RAE1 RNA Export 1 Homolog (S. Pombe) (RAE1))

Alternative Name: RAE1

Background: Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Cellular Localization: Cytoplasm , Nucleus

Molecular Weight: 41 kDa

Gene ID: 8480

Pathways: SARS-CoV-2 Protein Interactome