AKAP12 antibody (C-Term)

Catalog No. ABIN2856350

The Rabbit Polyclonal anti-AKAP12 antibody has been validated for WB, IF, ICC and IHC (p). It is suitable to detect AKAP12 in samples from Human.

Quick Overview for AKAP12 antibody (C-Term) (ABIN2856350)

Target: AKAP12 (A Kinase (PRKA) Anchor Protein 12 (AKAP12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP12 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AKAP12 Antibody

Binding Specificity: C-Term

Characteristics: Rabbit polyclonal antibody to AKAP12 (A kinase (PRKA) anchor protein (gravin) 12)
AKAP12 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AKAP12. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.73 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for AKAP12

Target: AKAP12 (A Kinase (PRKA) Anchor Protein 12 (AKAP12))

Alternative Name: AKAP12

Background: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Cellular Localization: Cytoplasm , cell cortex , cytoskeleton

Molecular Weight: 191 kDa

Gene ID: 9590

Pathways: cAMP Metabolic Process