CBFB antibody

Catalog No. ABIN2856441

This anti-CBFB antibody is a Rabbit Polyclonal antibody detecting CBFB in WB. Suitable for Human.

Quick Overview for CBFB antibody (ABIN2856441)

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBFB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CBFB Antibody

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to v (core-binding factor, beta subunit)
CBFb antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CBFb. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CBFB

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Alternative Name: core-binding factor subunit beta

Background: The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit, it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Nucleus

Molecular Weight: 22 kDa

Gene ID: 865

UniProt: Q13951

Pathways: Regulation of Lipid Metabolism by PPARalpha