SPOP-B antibody (Center)

Catalog No. ABIN2856462

This anti-SPOP-B antibody is a Rabbit Polyclonal antibody detecting SPOP-B in WB and IHC. Suitable for Human and Mouse.

Quick Overview for SPOP-B antibody (Center) (ABIN2856462)

Target: SPOP-B (Speckle-Type POZ Protein (SPOP-B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPOP-B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SPOP-B Antibody

Binding Specificity: Center

Cross-Reactivity: Rat (Rattus), Xenopus tropicalis, Rhesus Monkey, Cow (Bovine)

Cross-Reactivity (Details): Rat (100 %), Xenopus Tropicalis (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to SPOP (speckle-type POZ protein)
SPOP antibody [N1C1]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human SPOP. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Immunohistochemistry Assay-dependent dilution Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceImmunohistochemistryAssay-dependent dilution Western blot1:500-1:3000*

Comment:

Positive Control: 293T , Raji , mouse brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SPOP-B

Target: SPOP-B (Speckle-Type POZ Protein (SPOP-B))

Alternative Name: SPOP

Background: This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.

Cellular Localization: Nucleus

Molecular Weight: 42 kDa

Gene ID: 8405