Doublecortin antibody

Catalog No. ABIN2856489

Product Details anti-Doublecortin Antibody

Target: Doublecortin (DCX)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Doublecortin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to Doublecortin (doublecortex, lissencephaly, X-linked (doublecortin))
Doublecortin antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Doublecortin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:50-1:200. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: human DCX-transfected 293T

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.76 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Doublecortin

Target: Doublecortin (DCX)

Alternative Name: doublecortin (DCX Products)

Synonyms: DCX antibody, DBCN antibody, DC antibody, LISX antibody, SCLH antibody, XLIS antibody, Dbct antibody, 18C15.5 antibody, doublecortin antibody, DCX antibody, Dcx antibody

Background: In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene.

Cellular Localization: Cytoplasm

Molecular Weight: 49 kDa

Gene ID: 1641

UniProt: O43602