TUBB1 antibody

Catalog No. ABIN2856496

This Rabbit Polyclonal antibody specifically detects TUBB1 in WB, IF, ICC, IP, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for TUBB1 antibody (ABIN2856496)

Target: TUBB1 (Tubulin, beta 1 (TUBB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TUBB1 Antibody

Cross-Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to beta Tubulin (tubulin, beta 1)
beta Tubulin antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , HepG2 , NIH-3T3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for TUBB1

Target: TUBB1 (Tubulin, beta 1 (TUBB1))

Alternative Name: tubulin beta 1 class VI

Background: Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, platelet formation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that is wound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in platelets and megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 [PubMed 3782288], Schulze et al., 2004 [PubMed 15315966]).[supplied by OMIM]

Molecular Weight: 50 kDa

Gene ID: 81027

UniProt: Q9H4B7

Pathways: Microtubule Dynamics