UNC13D antibody (C-Term)

Catalog No. ABIN2856569

The Rabbit Polyclonal anti-UNC13D antibody is suitable to detect UNC13D in samples from Human. It has been validated for WB, IHC (p), IF and ICC.

Quick Overview for UNC13D antibody (C-Term) (ABIN2856569)

Target: UNC13D (Unc-13 Homolog D (UNC13D))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UNC13D antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-UNC13D Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to Munc 13-4 (unc-13 homolog D (C. elegans))
Munc 13-4 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Munc 13-4. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat , K562

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for UNC13D

Target: UNC13D (Unc-13 Homolog D (UNC13D))

Alternative Name: unc-13 homolog D

Background: This gene encodes a protein that is a member of the UNC13 family, containing similar domain structure as other family members but lacking an N-terminal phorbol ester-binding C1 domain present in other Munc13 proteins. The protein appears to play a role in vesicle maturation during exocytosis and is involved in regulation of cytolytic granules secretion. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis type 3, a genetically heterogeneous, rare autosomal recessive disorder.

Cellular Localization: Cytoplasm , Membrane, Peripheral membrane protein

Molecular Weight: 123 kDa

Gene ID: 201294

UniProt: Q70J99

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process