TJP1 antibody (N-Term)

Catalog No. ABIN2856624

This anti-TJP1 antibody is a Rabbit Polyclonal antibody detecting TJP1 in WB, IP, IF and ICC. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for TJP1 antibody (N-Term) (ABIN2856624)

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TJP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-TJP1 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to ZO-1 (tight junction protein 1 (zona occludens 1))
ZO-1 antibody [N1N2], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.22 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-TJP1 antibody (ABIN2856624)

Zhang, Heruth, Wu, Zhang, Nsumu, Shortt, Li, Jiang, Wang, Friesen, Li, Ye: "Functional characterization of SLC26A3 c.392C>G (p.P131R) mutation in intestinal barrier function using CRISPR/CAS9-created cell models." in: Cell & bioscience, Vol. 9, pp. 40, (2019) (PubMed).

Target Details for TJP1

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Alternative Name: tight junction protein 1

Background: This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene.

Cellular Localization: Cell membrane, Peripheral membrane protein, Cytoplasmic side , Cell junction , tight junction

Molecular Weight: 195 kDa

Gene ID: 7082

UniProt: Q07157

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization