HMGCL antibody (full length)

Catalog No. ABIN2856650

The Rabbit Polyclonal anti-HMGCL antibody has been validated for WB, ICC, IHC (p) and IF. It is suitable to detect HMGCL in samples from Human, Mouse and Rat.

Quick Overview for HMGCL antibody (full length) (ABIN2856650)

Target: HMGCL (3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGCL antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-HMGCL Antibody

Binding Specificity: full length

Characteristics: Rabbit Polyclonal antibody to HMGCL (3-hydroxymethyl-3-methylglutaryl-CoA lyase)
HMGCL antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Full length human HMGCL Recombinant protein.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:1000-1:10000*

Comment:

Positive Control: H1299 , mouse liver , rat liver

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for HMGCL

Target: HMGCL (3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL))

Alternative Name: HMGCL

Background: The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Mitochondrion matrix

Molecular Weight: 34 kDa

Gene ID: 3155