HMGCL antibody (AA 1-325)

Catalog No. ABIN561291

Cited in 2+ publications. The Mouse Monoclonal anti-HMGCL antibody (Clone 4F4-D1) (ABIN561291) specifically detects HMGCL in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for HMGCL antibody (AA 1-325) (ABIN561291)

Target: HMGCL (3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HMGCL antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 4F4-D1

Product Details anti-HMGCL Antibody

Binding Specificity: AA 1-325

Purpose: Mouse monoclonal antibody raised against a full length recombinant HMGCL.

Sequence: MAAMRKALPR RLVGLASLRA VSTSSMGTLP KRVKIVEVGP RDGLQNEKNI VSTPVKIKLI DMLSEAGLSV IETTSFVSPK WVPQMGDHTE VLKGIQKFPG INYPVLTPNL KGFEAAVAAG AKEVVIFGAA SELFTKKNIN CSIEESFQRF DAILKAAQSA NISVRGYVSC ALGCPYEGKI SPAKVAEVTK KFYSMGCYEI SLGDTIGVGT PGIMKDMLSA VMQEVPLAAL AVHCHDTYGQ ALANTLMALQ MGVSVVDSSV AGLGGCPYAQ GASGNLATED LVYMLEGLGI HTGVNLQKLL EAGNFICQAL NRKTSSKVAQ ATCKL

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: HMGCL (AAH10570, 1 a.a. ~ 325 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-HMGCL antibody (ABIN561291)

Arnedo, Menao, Puisac, Teresa-Rodrigo, Gil-Rodríguez, López-Viñas, Gómez-Puertas, Casals, Casale, Hegardt, Pié: "Characterization of a novel HMG-CoA lyase enzyme with a dual location in endoplasmic reticulum and cytosol." in: Journal of lipid research, Vol. 53, Issue 10, pp. 2046-56, (2012) (PubMed).

Puisac, Arnedo, Casale, Ribate, Castiella, Ramos, Ribes, Pérez-Cerdá, Casals, Hegardt, Pié: "Differential HMG-CoA lyase expression in human tissues provides clues about 3-hydroxy-3-methylglutaric aciduria." in: Journal of inherited metabolic disease, Vol. 33, Issue 4, pp. 405-10, (2010) (PubMed).

Target Details for HMGCL

Target: HMGCL (3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL))

Alternative Name: HMGCL

Background: Full Gene Name: 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
Synonyms: HL

Gene ID: 3155