UNC13B antibody (Internal Region)

Catalog No. ABIN2856793

This Rabbit Polyclonal antibody specifically detects UNC13B in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for UNC13B antibody (Internal Region) (ABIN2856793)

Target: UNC13B (Unc-13 Homolog B (UNC13B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UNC13B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-UNC13B Antibody

Binding Specificity: Internal Region

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine)

Cross-Reactivity (Details): Mouse (99 %), Rat (98 %), Bovine (99 %)

Characteristics: Rabbit Polyclonal antibody to UNC13B (unc-13 homolog B (C. elegans))
UNC13B antibody [N3C1], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human UNC13B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , H1299 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for UNC13B

Target: UNC13B (Unc-13 Homolog B (UNC13B))

Alternative Name: UNC13B

Background: This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis.

Cellular Localization: Cytoplasm (By similarity) , Membrane, Peripheral membrane protein (By similarity) , Cell membrane (By similarity) , Cell junction , synapse (By similarity)

Molecular Weight: 181 kDa

Gene ID: 10497

Pathways: Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis