NHEJ1 antibody

Catalog No. ABIN2857060

This Rabbit Polyclonal antibody specifically detects NHEJ1 in WB, IF, IHC (p), IP and ICC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for NHEJ1 antibody (ABIN2857060)

Target: NHEJ1 (Nonhomologous End-Joining Factor 1 (NHEJ1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NHEJ1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-NHEJ1 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to XLF (nonhomologous end-joining factor 1)
XLF antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human XLF. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-NHEJ1 antibody (ABIN2857060)

Vu, Bonucci, Brenière, Cisneros-Aguirre, Pelupessy, Wang, Carlier, Bouvignies, Cortes, Aggarwal, Blackledge, Gueroui, Belle, Stark, Modesti, Ferrage: "Multivalent interactions of the disordered regions of XLF and XRCC4 foster robust cellular NHEJ and drive the formation of ligation-boosting condensates in vitro." in: Nature structural & molecular biology, Vol. 31, Issue 11, pp. 1732-1744, (2024) (PubMed).

Target Details for NHEJ1

Target: NHEJ1 (Nonhomologous End-Joining Factor 1 (NHEJ1))

Alternative Name: non-homologous end joining factor 1

Background: Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.

Cellular Localization: Nucleus

Molecular Weight: 33 kDa

Gene ID: 79840

UniProt: Q9H9Q4

Pathways: DNA Damage Repair