LIG4 antibody

Catalog No. ABIN2857063

This Rabbit Polyclonal antibody specifically detects LIG4 in WB and IHC (p). It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for LIG4 antibody (ABIN2857063)

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-LIG4 Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit polyclonal antibody to DNA ligase IV (ligase IV, DNA, ATP-dependent)
DNA ligase IV antibody [N2C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DNA ligase IV. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Molt-4

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.65 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-LIG4 antibody (ABIN2857063)

Ly, Teitz, Kim, Shoshani, Skaletsky, Fachinetti, Page, Cleveland: "Selective Y centromere inactivation triggers chromosome shattering in micronuclei and repair by non-homologous end joining." in: Nature cell biology, Vol. 19, Issue 1, pp. 68-75, (2017) (PubMed).

Zhu, Fisher, Bessho, Peng: "Protein phosphatase 1 and phosphatase 1 nuclear targeting subunit-dependent regulation of DNA-dependent protein kinase and non-homologous end joining." in: Nucleic acids research, Vol. 45, Issue 18, pp. 10583-10594, (2017) (PubMed).

Target Details for LIG4

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Alternative Name: DNA ligase 4

Background: The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

Cellular Localization: Nucleus

Molecular Weight: 104 kDa

Gene ID: 3981

UniProt: P49917

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response