Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Specificity
This AB is 100 % conserved in Mouse and Rat, 91% in Golden hamster, 83% in Rabbit and Pig, and 75% in Human and Canine.
Cross-Reactivity (Details)
This rat D3R peptide sequence is 88 % conserved in mouse and 63 % with human/monkey D3R receptors (1-2). No significant sequence homology with other dopamine receptors (D1, D2, D4 or D5).
Immunogen
Dopamine D3 receptor antibody was raised in rabbit using a 19 amino acid peptide of human D3 R as the immunogen.
Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. D3 is encoded by the Dopamine receptor D3 gene (DRD3). Maximum expression of dopamine D3 receptors is noted in the islands of Calleja and nucleus accumbens.