HYOU1 antibody (Atto 488)

Catalog No. ABIN2868630

The Mouse Monoclonal anti-HYOU1 antibody has been validated for WB, IF, ICC. It is suitable to detect HYOU1 in samples from Human.

Quick Overview for HYOU1 antibody (Atto 488) (ABIN2868630)

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HYOU1 antibody is conjugated to Atto 488

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 6E3-2C3

Product Details anti-HYOU1 Antibody (Atto 488)

Specificity: Detects ~170 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Recombinant Full length GRP170 Protein

Isotype: IgG2b

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2868630 was sufficient for detection of GRP170 in 20 μg of HEK293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for HYOU1

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Alternative Name: GRP170

Background: GRP170, also known as ORP150, is the largest member of glucose-regulated Antibodys, and acts as a human chaperone Antibody. It is thought to play an important role in Antibody folding and secretion in the ER. Suppression of the Antibody is associated with accelerated apoptosis, therefore having an important cryoprotective role in hypoxia-induced cellular pertubation. This cryopotective role has led to an anti-tumor immune response, which will hopefully lead to therapeutic immunizations against cancers (1). GRP170 has also been shown to bind with dendritic cells and provide the danger signals to induce anti-tumor immune responses (2).

Gene ID: 10525

NCBI Accession: NP_006389

UniProt: Q9Y4L1

Pathways: ER-Nucleus Signaling, SARS-CoV-2 Protein Interactome