Beclin 1 antibody (C-Term) (HRP)

Catalog No. ABIN2868770

This Rabbit Polyclonal antibody specifically detects Beclin 1 in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for Beclin 1 antibody (C-Term) (HRP) (ABIN2868770)

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Beclin 1 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Beclin 1 Antibody (HRP)

Binding Specificity: C-Term

Specificity: Predicted molecular weight at ~51 kDa.

Cross-Reactivity: Human, Rat

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide from the C-terminus of human Beclin 1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN2868770 was sufficient for detection of Beclin1 on 293T lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Alternative Name: Beclin 1

Background: Beclin 1 is the mammalian ortholog of the yeast autophagy -related gene Atg6. It regulates autophagy and has an inportant role in development, tumorigenesis and neurodegeneration (1). Beclin 1 is localized within cytoplasmic structures including the mitochondria, yet overexpression reveals some nuclear staining (2). Researchers have found that schizonphrenia is associated with low levels of Beclin-1 in the hippocampus (3). It may also protect against infection by a neuorvirulent train of Sindbis virus (4).

Gene ID: 8678

NCBI Accession: NP_001300927

UniProt: Q14457

Pathways: Autophagy