Gelsolin antibody

Catalog No. ABIN2878466

The Mouse Monoclonal anti-Gelsolin antibody (ABIN2878466) specifically detects Gelsolin in IHC, ELISA and IHC (p). The antibody is reactive with Human samples.

Quick Overview for Gelsolin antibody (ABIN2878466)

Target: Gelsolin (GSN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Gelsolin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Gelsolin Antibody

Specificity: Recognizes human gelsolin.

Purification: Protein A/G purified

Immunogen: Gelsolin purified from human plasma.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (1:2000), IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA: 1:2000 applies to gelsolin presented on a coat of polyclonal capture antibody. Note: Does not work as capture antibody in sandwich ELISA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 15 mM sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Gelsolin

Target: Gelsolin (GSN)

Alternative Name: GSN / Gelsolin

Background: Name/Gene ID: GSN

Synonyms: GSN, ADF, AGEL, Brevin, Actin-depolymerizing factor, Gelsolin precursor, plasma, Gelsolin

Gene ID: 2934

UniProt: P06396

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy