Interferon gamma antibody (Biotin)

Catalog No. ABIN288304

This Goat Polyclonal antibody specifically detects Interferon gamma in WB and ELISA. It exhibits reactivity toward Rat.

Quick Overview for Interferon gamma antibody (Biotin) (ABIN288304)

Target: Interferon gamma (IFNG)

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Interferon gamma antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-Interferon gamma Antibody (Biotin)

Immunogen: IFN gamma antibody (biotin) was raised in goat using highly pure recombinant rat IFN-gamma as the immunogen.

Application Details

Application Notes: ELISA: 0.25-1 µg/mL, WB: 0.1-0.2 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFN gamma

Background: IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-gamma is species-specific and is biologically active only in human and primate cells.

Molecular Weight: 15.6 kDa (predicted detection band MW)

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy