Ig antibody (Light Chain) (HRP)

Catalog No. ABIN288438

This anti-Ig antibody is a Goat Polyclonal antibody detecting Ig in ELISA, WB, IHC and ICC. Suitable for Mouse.

Quick Overview for Ig antibody (Light Chain) (HRP) (ABIN288438)

Target: Ig

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Ig antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-Ig Antibody (HRP)

Binding Specificity: Light Chain

Specificity: This antibody reacts with Human, Mouse, and Rat. This antibody does not cross-react with GLUT-4.

Cross-Reactivity (Details): This Antibody may cross react with lambda from other species1 ml@ 1 mg/ml

Purification: Affinity chromatography purified

Isotype: IgG

Application Details

Application Notes: ELISA: 1:10,000-1:100,000, ICC: 1:200-1:500, IHC: 1:200-1:500, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: 50 mM Hepes, pH 7.1, with 0.0 NaCl, 0.2 % BSA, and 0.01 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Dilute only prior to immediate use.

Storage: 4 °C

Target Details for Ig

Target: Ig

Background: There are two types of light chain in mammals, the kappa chain, encoded by the immunoglobulin kappa locus on chromosome 2, and the lambda chain, encoded by the immunoglobulin lambda locus on chromosome 22. Antibodies are produced by B lymphocytes, each expressing only one class of light chain. Once set, light chain class remains fixed for the life of the B lymphocyte.