Collagen Type V antibody

Catalog No. ABIN2888314

The Mouse Monoclonal anti-Collagen Type V antibody has been validated for WB, ELISA, IHC. It is suitable to detect Collagen Type V in samples from Human, Dog, Sheep.

Quick Overview for Collagen Type V antibody (ABIN2888314)

Target: Collagen Type V (COL5) (Collagen, Type V (COL5))

Reactivity: Human, Dog, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Collagen Type V antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Collagen Type V Antibody

Specificity: Highly specific for type V collagen. It has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and Western Blot. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). Shows reactivity with human, canine, bovine, sheep, porcine and kangaroo type V collagens by ELISA and Western Blot. Does not show reactivity with mouse, rat, guinea pig and chicken type V collagens by ELISA and Western Blot. Can be used for detection of collagens by ELISA. Has been used successfully for immunohistology on frozen unfixed sections of human, bovine and dog skin, of fetal bovine cornea, and of new dog tissue associated with a biomaterial implant. If fixation of tissue is required, acetone or ethanol is recommended. Binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.

Purification: Protein A/G purified

Immunogen: Acid-digested pepsin soluble canine type V collagen.

Type of Immunogen: Protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA (1:50), IHC, WB

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry. ELISA: 1:50.

Comment:

Target Species of Antibody: Dog

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for Collagen Type V

Target: Collagen Type V (COL5) (Collagen, Type V (COL5))

Alternative Name: Collagen Type V