IL-6 antibody

Catalog No. ABIN289357

This Rabbit Polyclonal antibody specifically detects IL-6 in WB, ELISA and Inhibition. It exhibits reactivity toward Mouse.

Quick Overview for IL-6 antibody (ABIN289357)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Blocking Antibody (Inhibition)

Product Details anti-IL-6 Antibody

Purification: Purified

Immunogen: IL6 antibody was raised in rabbit using highly pure recombinant murine IL-6 as the immunogen.

Application Details

Application Notes: ELISA: 0.5-2 µg/mL, Inhibition: 0.24-0.36 µg/mL, WB: 0.1-0.2 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration > 0.2 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.4.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL6

Background: IL6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL6 signals through the IL6 receptor system that consists of two chains, IL6R alpha and gp130. Mouse IL6 is inactive on human cells, while both human and Mouse are equally active on Mouse cells.

Molecular Weight: 21.7 kDa (predicted detection band MW)

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome