Dopamine d2 Receptor antibody (AA 9-21)

Catalog No. ABIN289417

Product Details anti-Dopamine d2 Receptor Antibody

Target: Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))

Binding Specificity: AA 9-21

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dopamine d2 Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity (Details): This rat antigenic peptide is 100 % conserved in rat, pig, bovine, 92 % in mouse, and 78 % in human D1 (D1A/DRD1). No significant sequence homology exist with other dopamine receptors (D1B, D2-D5).

Purification: Affinity chromatography purified

Immunogen: Dopamine receptor 2 antibody was raised in rabbit using a 13 amino acid peptide of rat D1 R as the immunogen.

Isotype: IgG

Application Details

Application Notes: ELISA: 0.5-1 µg/mL, IHC: 2-10 µg/mL, WB: 1-10 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

Dopamine Receptor 2 Blocking peptide, catalog no. 30R-AD007, is also available for use as a blocking control in assays to test for the specificity of this Dopamine Receptor 2 antibody.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from PBS, with 0.1 % BSA, and 0.05 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for Dopamine d2 Receptor

Target: Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))

Alternative Name: Dopamine Receptor 2 (DRD2 Products)

Background: Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. The D1 subtype is the most abundant dopamine receptor in the central nervous system. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events.

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity