IL-10 antibody

Catalog No. ABIN289469

The Rabbit Polyclonal anti-IL-10 antibody has been validated for WB, ELISA and Inhibition. It is suitable to detect IL-10 in samples from Mouse.

Quick Overview for IL-10 antibody (ABIN289469)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Blocking Antibody (Inhibition)

Product Details anti-IL-10 Antibody

Purification: Purified

Immunogen: IL10 antibody was raised in rabbit using highly pure recombinant murine IL-10 as the immunogen.

Application Details

Application Notes: ELISA: 0.5-2 µg/mL, Inhibition: 0.043-0.064 µg/mL, WB: 0.1-0.2 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of > 0.2 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from 0.5 x PBS, pH 7.4.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL10

Background: IL10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL10 inhibits the expression of proinflammatory cytokines such as IL1 and TNFa. Like IL4, IL10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL10 active on Mouse cells, but Mouse IL10 is inactive on human cells.

Molecular Weight: 18.7 kDa (predicted detection band MW)

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints