Ret Proto-Oncogene antibody (Extracellular Domain)

Catalog No. ABIN289533

This anti-Ret Proto-Oncogene antibody is a Goat Polyclonal antibody detecting Ret Proto-Oncogene in WB, ELISA, IHC (p) and IHC (fro). Suitable for Mouse. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Ret Proto-Oncogene antibody (Extracellular Domain) (ABIN289533)

Target: Ret Proto-Oncogene (RET)

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Ret Proto-Oncogene antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Ret Proto-Oncogene Antibody

Binding Specificity: Extracellular Domain

Purification: Purified

Immunogen: Ret antibody was raised in goat using NSO derived murine ret extracellular domain as the immunogen.

Application Details

Application Notes: ELISA: 0.5-1 μg/mL, IHC-F: 5 μg/mL, IHC-P: 10-15 μg/mL, WB: 0.1-0.2 μg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with PBS.

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2 containing 5 % trehalose.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: -80 °C

Storage Comment: Aliquot and store at -70 °C or lower.

References for anti-Ret Proto-Oncogene antibody (ABIN289533)

Gay, Valenta, Herr, Paratore-Hari, Basler, Sommer: "Distinct adhesion-independent functions of β-catenin control stage-specific sensory neurogenesis and proliferation." in: BMC biology, Vol. 13, pp. 24, (2015) (PubMed).

Aron, Klein, Pham, Kramer, Wurst, Klein: "Pro-survival role for Parkinson's associated gene DJ-1 revealed in trophically impaired dopaminergic neurons." in: PLoS biology, Vol. 8, Issue 4, pp. e1000349, (2010) (PubMed).

Target Details for Ret Proto-Oncogene

Target: Ret Proto-Oncogene (RET)

Alternative Name: Ret

Background: The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicompetent receptor complex in conjunction with other membrane-bound ligand-binding GDNF family receptors. Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Alterations in the corresponding Ret gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma and a congenital developmental disorder known as Hirschsprung’s disease. The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr905 dramatically inhibits Ret autophosphorylation activity.

Pathways: RTK Signaling, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation