UNC13D antibody (AA 1079-1090)

Catalog No. ABIN290188

This Goat Polyclonal antibody specifically detects UNC13D in ELISA, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for UNC13D antibody (AA 1079-1090) (ABIN290188)

Target: UNC13D (Unc-13 Homolog D (UNC13D))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This UNC13D antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UNC13D Antibody

Binding Specificity: AA 1079-1090

Specificity: Human UNC13D / Munc13-4.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset (92%) Hamster (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KQASQHALRPAP from the C-terminus of human UNC13D / Munc13-4 (NP_954712.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset (92%), Hamster (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for UNC13D

Target: UNC13D (Unc-13 Homolog D (UNC13D))

Alternative Name: UNC13D

Background: Name/Gene ID: UNC13D

Synonyms: UNC13D, HLH3, Unc-13 homolog D (C. elegans), Protein unc-13 homolog D, HPLH3, Munc13-4

Gene ID: 201294

NCBI Accession: NP_954712

UniProt: Q70J99

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process