LSD1 antibody (AA 100-150)

Catalog No. ABIN290327

The Rabbit Polyclonal anti-LSD1 antibody is suitable to detect LSD1 in samples from Human, Mouse, Rat, Monkey, Dog, Macaque and Zebrafish (Danio rerio). It has been validated for WB, IHC and IHC (p).

Quick Overview for LSD1 antibody (AA 100-150) (ABIN290327)

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Reactivity: Human, Mouse, Rat, Monkey, Dog, Macaque, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LSD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LSD1 Antibody

Binding Specificity: AA 100-150

Specificity: The amino acid sequence used as immunogen is 100 % homologous in human (isoform b), mouse, rat, dog, zebrafish and rhesus monkey.

Purification: Immunoaffinity purified

Immunogen: A portion of amino acids 100-150 of human LSD1.

Type of Immunogen: Protein fragment

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.

Target Details for LSD1

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Alternative Name: KDM1A / LSD1

Background: Name/Gene ID: KDM1A

Synonyms: KDM1A, BHC110, KDM1, LSD1, AOF2, KIAA0601

Gene ID: 23028

UniProt: O60341

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, Warburg Effect