GPR109B antibody (AA 1-50)

Catalog No. ABIN290337

The Rabbit Polyclonal anti-GPR109B antibody (ABIN290337) specifically detects GPR109B in WB, IHC (p) and IHC. The antibody is reactive with Human, Dog and Hamster samples.

Quick Overview for GPR109B antibody (AA 1-50) (ABIN290337)

Target: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Reactivity: Human, Dog, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR109B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Product Details anti-GPR109B Antibody

Binding Specificity: AA 1-50

Specificity: A portion of amino acids 1-50 of human GPR109B.

Purification: Immunoaffinity purified

Immunogen: A portion of amino acids 1-50of human GPR109B.

Type of Immunogen: Protein fragment

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for GPR109B

Target: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Alternative Name: HCAR3 / GPR109B / HM74

Background: Name/Gene ID: HCAR3
Subfamily: Nicotinic Acid
Family: GPCR

Synonyms: HCAR3, GPR109B, GTP-binding protein, NIACR2, HM74, HM74b, Nicotinic acid receptor 2, Putative chemokine receptor, Niacin receptor 2, Puma-g, HCA3, PUMAG

Gene ID: 8843

UniProt: P49019